Journal: Matrix Biology Plus

Article Title: Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis development

doi: 10.1016/j.mbplus.2023.100135

Figure Lengend Snippet: Versican ( VCAN ) expression is regulated by cytokines and mechanical stretch in cardiac cells. (A) mRNA expression of versican was upregulated by TGF-β1, and downregulated by TNF-α, and IL-1β in cardiac fibroblasts, and (B) in addition by IL-4 in cardiomyocytes compared to control (Control, n = 3). (C, D) Versican and α-SMA ( ACTA2 ) mRNA expression were increased after biaxial strain of 10% (stretch) for 24 h at 1 Hz frequency in fibroblasts compared to non-stretched cells (Control, n = 9). Gene expression is presented as values relative to Control. Bar graphs represent mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test (A, B) and Student’s t -test (C, D) were used for statistical analysis. P values < 0.05 were considered statistically significant. *P < 0.05 Control vs. cytokine treatment or stretch.

Article Snippet: Fetal human cardiac fibroblasts (CFs) (Cell Applications, CA, #306-05F) and primary human cardiomyocytes (CMs) (PromoCell, Germany, #C-12810) were used for in vitro experiments.

Techniques: Expressing, Control, Gene Expression

Journal: International Journal of Ophthalmology

Article Title: Effect of zymosan on the expression and function of the gap-junction protein connexin 43 in human corneal fibroblasts

doi: 10.18240/ijo.2021.03.02

Figure Lengend Snippet: A: After exposure of HCFs to various concentrations of zymosan, the expression of Cx43 was examined by Western blot analysis; B: The immunoblots were subjected to densitometric analysis in order to determine the band intensity. The error bars represent the standard deviation. aP<0.05 (Dunnett's multiple comparison test) versus the control (no zymosan).

Article Snippet: Culture of Human Corneal Fibroblasts HCFs were purchased from ScienCell Research Laboratories (cat. no. #6520; Carlsbad, CA, USA) and cultured in MEM containing 10% FBS (5% CO 2 , 37°C).

Techniques: Expressing, Western Blot, Standard Deviation, Comparison, Control

Journal: International Journal of Ophthalmology

Article Title: Effect of zymosan on the expression and function of the gap-junction protein connexin 43 in human corneal fibroblasts

doi: 10.18240/ijo.2021.03.02

Figure Lengend Snippet: A: After the exposure of HCFs to zymosan (600 µg/mL) for the indicated time, the expression of Cx43 was examined by Western blot analysis; B: The immunoblots were subjected to densitometric analysis in order to determine the band intensity. The error bars represent the standard deviation. aP<0.05 (Dunnett's multiple comparison test) versus the control (no zymosan).

Article Snippet: Culture of Human Corneal Fibroblasts HCFs were purchased from ScienCell Research Laboratories (cat. no. #6520; Carlsbad, CA, USA) and cultured in MEM containing 10% FBS (5% CO 2 , 37°C).

Techniques: Expressing, Western Blot, Standard Deviation, Comparison, Control

Journal: International Journal of Ophthalmology

Article Title: Effect of zymosan on the expression and function of the gap-junction protein connexin 43 in human corneal fibroblasts

doi: 10.18240/ijo.2021.03.02

Figure Lengend Snippet: After HCFs were incubated with or without zymosan (600 µg/mL) for 24h, the Cx43 mRNA level in the cells was then determined by qRT-PCR analysis. The error bars represent the standard deviation. aP<0.05 (Student's t-test) versus the control (no zymosan).

Article Snippet: Culture of Human Corneal Fibroblasts HCFs were purchased from ScienCell Research Laboratories (cat. no. #6520; Carlsbad, CA, USA) and cultured in MEM containing 10% FBS (5% CO 2 , 37°C).

Techniques: Incubation, Quantitative RT-PCR, Standard Deviation, Control

Journal: International Journal of Ophthalmology

Article Title: Effect of zymosan on the expression and function of the gap-junction protein connexin 43 in human corneal fibroblasts

doi: 10.18240/ijo.2021.03.02

Figure Lengend Snippet: A: After HCFs were treated with PD98059, SB203580, or JNK inhibitor II (at 1, 3, and 10 µmol/L), respectively, in the absence or presence of zymosan (600 µg/mL), the expression of Cx43 was then examined by Western blot analysis; B: Densitometric analysis was performed for the immunoblots to determine the band intensity. The error bars represent the standard deviation. aP<0.05 (Dunnett's test) versus the control (no zymosan); bP<0.05 (Dunnett's test) versus the corresponding control (zymosan only).

Article Snippet: Culture of Human Corneal Fibroblasts HCFs were purchased from ScienCell Research Laboratories (cat. no. #6520; Carlsbad, CA, USA) and cultured in MEM containing 10% FBS (5% CO 2 , 37°C).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: International Journal of Ophthalmology

Article Title: Effect of zymosan on the expression and function of the gap-junction protein connexin 43 in human corneal fibroblasts

doi: 10.18240/ijo.2021.03.02

Figure Lengend Snippet: A: HCFs were treated with IKK2 inhibitor IV (1, 3, and 10 µmol/L) in the absence or presence of zymosan (600 µg/mL). The expression of Cx43 was then examined by Western blot analysis. B: The immunoblots were subjected to densitometric analysis in order to determine the band intensity. The error bars represent the standard deviation. aP<0.05 (Dunnett's test) versus the control (no zymosan). bP<0.05 (Dunnett's test) versus the corresponding control (zymosan only).

Article Snippet: Culture of Human Corneal Fibroblasts HCFs were purchased from ScienCell Research Laboratories (cat. no. #6520; Carlsbad, CA, USA) and cultured in MEM containing 10% FBS (5% CO 2 , 37°C).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: International Journal of Ophthalmology

Article Title: Effect of zymosan on the expression and function of the gap-junction protein connexin 43 in human corneal fibroblasts

doi: 10.18240/ijo.2021.03.02

Figure Lengend Snippet: HCFs were treated with the ERK inhibitor PD98059, JNK inhibitor II, or IKK2 inhibitor IV (10 µmol/L), respectively, in the absence or presence of zymosan (600 µg/mL). A scrape-loading assay with Lucifer yellow was used to measure the GJIC activity. A: Representative fluorescence microscopy images of fixed cells from different treatments (scale bar, 100 µm); B: The maximum distances from the scrape to dye fluorescence were quantified to show the GJIC activity. The error bars represent the standard deviation. aP<0.05 (Dunnett's test) versus the control (no zymosan). bP<0.05 (Dunnett's test) versus the corresponding control (zymosan only).

Article Snippet: Culture of Human Corneal Fibroblasts HCFs were purchased from ScienCell Research Laboratories (cat. no. #6520; Carlsbad, CA, USA) and cultured in MEM containing 10% FBS (5% CO 2 , 37°C).

Techniques: Activity Assay, Fluorescence, Microscopy, Standard Deviation, Control